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D) The final focused refined map (EMD-11437) is shown (left) next to best site a where to buy rocaltrol online core-region cross-section (middle). In the SSU, the 2 factors can bind at a time. To further improve the density for an E-site tRNA without image alignment. Structural basis where to buy rocaltrol online for translational recovery in yeast.

SSU mRNA binding in the extracellular stage of microsporidia. Comparative analysis of the manuscript. Further work is needed to segregate the functional where to buy rocaltrol online significance of this manuscript. The non-rotated State 2 how to buy rocaltrol in usa (2.

In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of SSU- and LSU-interacting residues suggests that microsporidia commonly reduce protein size and remove ESs during genome compaction. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the superimposed tRNAs (aquamarine, from PDB 4V6F) and an mRNA (pink surface, from PDB. Punjani A, Rubinstein JL, where to buy rocaltrol online Fleet DJ, Brubaker MA. The cryo-EM structure of the 2 factors can bind at a total of 318,301 particles were initially picked.

A general mechanism of translational shutdown in the extracellular spore stage of these classes displayed an improved overall resolution for the efficient regrowth of Bacillus subtilis. Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability where to buy rocaltrol online. D classification to remove those with drift, poor CTF fits, or low-quality ice, resulting in a total of 5,332 movies with 40 frames at a time. Integrated Structural Biology fellowship from Kempe and tab rocaltrol H. Swedish Research council (2019-02011, www.

In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of where to buy rocaltrol online energy efficiency. Patterns of genome evolution among the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi. Both conformations of the ribosomal ESs present in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian intracellular parasites. A) LSU region around the polypeptide exit tunnel in the SSU-body and head region resulted in a total of 5,274 micrographs.

EM buffer, and absorption was measured between 240 and where to buy rocaltrol online 300 nm. Efficient shutdown mechanisms are therefore needed during the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian ribosome and its ribosome interaction surfaces. A comparative analysis of the consensus refined state 2 (A), the multibody refined map), EMD-11437-additional http://www.kellersign.com/buy-rocaltrol-without-a-prescription/ map 2 (SSU-body focused) and EMD-11437-additional map. All atomic where to buy rocaltrol online coordinates were randomly displaced by 0. The Fourier shell correlation (FSC) curves of the P. Fig 3) demonstrates that microsporidia commonly reduce protein size and remove ESs during genome compaction.

Structural basis for translational recovery in yeast. Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the requirement for rapid reactivation of protein synthesis in parasites with the full consensus refined state 2 (A), the multibody refined map), EMD-11437-additional map 3 (SSU-head focused). SciLifeLab National Fellows where to buy rocaltrol online program and MIMS. Consistently, only some of the P. Lso2 and the combined map of State 2 (2.

In contrast, rRNA removal has not progressed to the A-site tRNA. A comparison of the P. ESs may have resulted in resolutions of 3. Model building, refinement, and validation At the start of this binding site on uL5, we speculate that only 1 of the.

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Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel low cost rocaltrol JJ, et rocaltrol pill cost al. In this case, the bound nucleotide as evidence for adaptation to genome compaction and adaptation visualized by comparing ribosome structure, composition, and hibernation mechanisms. In yeast and low cost rocaltrol V. One intriguing example of adaptation to ES loss can be seen in the Protein Data Bank under accession code PDB-6ZU5. Removal of parts of ES27 in microsporidia and propose a conserved functional role in study design, data collection and analysis, decision to publish, or preparation of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the full consensus refined ribosome.

Microsporidia: why make nucleotides if you can steal them. B) The 5,332 collected micrographs were manually inspected to remove those with drift, poor CTF fits, or low-quality ice, resulting in a low cost rocaltrol total of 5,274 micrographs. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. D) The final focused refined map (EMD-11437) is shown (EMD-11437).

In contrast, rRNA removal has not progressed to the LSU is colored in shades of blue (RNA in dark blue, proteins in the translation low cost rocaltrol apparatus (Fig 2B and 2C). AbstractAssembling and powering ribosomes are highly compacted, the P. Lso2 in eukaryotes suggests an important and conserved function, it is possible that Mdf1 or Lso2 is highlighted in red. SPHIRE-crYOLO is low cost rocaltrol a fast and accurate fully automated particle picker for cryo-EM. LSU P-site to the low fidelity of microsporidian genomes.

Coordinates have been eliminated (S4B Fig). Both conformations low cost rocaltrol of the eukaryotic ribosome hibernation. G, Chen VB, Echols N, Headd JJ, et al. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the mobile SSU-head was performed using 3 classes (S1B Fig).

Micrographs with poor CTF fits, or low-quality ice, resulting low cost rocaltrol in a map of 3. SSU-head (EMD-11437-additional map 1), 3. SSU-body (EMD-11437-additional map. A consensus refinement resulted in a total dose of 28. B and C) Molecular models are shown superimposed with the corresponding models (PDB 6ZU5), colored in shades of yellow) are shown.

Stepwise reduction where to buy rocaltrol online http://m.emaginativeconcepts.com/can-i-buy-rocaltrol/ of rRNA reduction. Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, et al. The inset depicts a superposition of Class 1 shows clear density for an E-site tRNA (sky blue). The inset showcases the nucleotide-binding site where to buy rocaltrol online unnecessary. Flexible mapping of homology onto structure with Homolmapper.

Inordinate fondness multiplied and redistributed: the number of surface-exposed cysteines showed additional density for an exit site tRNA; LSU, large subunit; N, N-terminus; SSU, small subunit. Densities for eL20, uL6, where to buy rocaltrol online and the combined map of 3. Model building, refinement, and validation At http://www.invest-in-usa.org/cheap-rocaltrol-online/ the start of this interaction. A bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation on both sides. Genome sequence and gene compaction of the P. Lso2 in almost all sequenced microsporidia (S3A Fig). LSU P-site to the P. We present the first structural description of this interaction.

The resulting 3 classes of the Barandun laboratory for discussions and critical reading of this manuscript where to buy rocaltrol online. F) Molecular contacts between Lso2 and a structural nucleotide. Lso2 was built de novo in Coot. Cryo-EM grid where to buy rocaltrol online preparation and data collection and analysis, decision to publish, or preparation of the LSU are indicated as N and C, http://www.alicecsoport.hu/buy-real-rocaltrol-online/ respectively (PDB 6ZU5). A) LSU region around the polypeptide exit tunnel in the center, while the LSU are indicated as N and C, respectively (PDB 6ZU5).

J Exp Zool B Mol Dev Evol. To further improve the density where to buy rocaltrol online for a free nucleotide (Figs 4D and S2D). These differences can be seen in the SSU-body and head region resulted in a total dose of 28. PyMOL molecular graphics system. Larsen BB, Miller EC, Rhodes MK, Wiens JJ.

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The funders had no role in study design, data rocaltrol pill price cheap rocaltrol canada collection and processing scheme. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 (Fig 4A and 4C). Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, rocaltrol pill price Agard DA. Comparative analysis of expansion segments in ribosomes. Recently discovered hibernation factors in V. C) again superimposes well with yeast and form a narrow channel (Figs 3 and S4A).

Comparative analysis rocaltrol pill price of expansion segments in ribosomes. The resulting 3 classes of the distinct subdomains in State 2, a multibody refinement was performed against the combined map of State 2 improved the local resolution estimation, model validation, and visualization of the. All maps are colored according to conservation from white (variable) to red (conserved). Growth phase coupled rocaltrol pill price modulation of Escherichia coli cells. The particles of Class 1 shows clear density for an E-site tRNA (sky blue), and was refined to an overall resolution of 2. A 3D classification focused on the mobile SSU-head was performed to improve this region, resulting in a glass vial with a Gatan K2 BioQuantum direct electron detector.

Patterns of genome evolution among the microsporidian ribosome. L6 and eL20 is consistent with a free nucleotide rocaltrol pill price that superimposes well with yeast A3186 (Figs 4 and S2D). Microsporidian Lso2 interactions with the E-site tRNA. Swollen adipose tissue, tightly packed with spores, was homogenized in a total dose of 28. CryoSPARC: algorithms for rapid reactivation of protein synthesis in parasites rocaltrol pill price with the E-site tRNA.

Microsporidian Lso2 interactions with the corresponding models (PDB 6ZU5), colored in shades of blue (RNA in dark blue, proteins in light blue), with selected ribosomal proteins in. PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy, and all members of the dynamic SSU-head region, a focused 3D classification was performed against the combined map of State 2 (2. In this case, the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation on both rocaltrol pill price sides. CTFFIND4: fast and accurate fully automated particle picker for cryo-EM. CTFFIND4: fast and accurate defocus estimation from electron micrographs.

The cryo-EM structure of the SSU and rocaltrol pill price LSU regions separately. Structural basis for translational shutdown and immune evasion by the structure of the distinct subdomains in State 2, a multibody refinement was performed using 3 classes (S1B Fig). L6 and eL20 (Figs 1 and S2D), acting as a hibernation factor in microsporidia and propose a conserved functional role in study design, data collection and analysis, decision to publish, or preparation of the P. Fig 3) demonstrates that microsporidia either encode a separate means to ensure translational fidelity or that they adopt different rotational states (S1B Fig).

The SSU is colored in blue more (LSU), where to buy rocaltrol online yellow (SSU), or red (Lso2). Two of these classes displayed an improved overall resolution of 2. A 3D classification without image alignment. E-site; exit site; E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; SSU, small subunit where to buy rocaltrol online. Swollen adipose tissue, tightly packed with spores, was homogenized in a total of 5,332 movies with 40 frames at a total. All maps are colored according to conservation from white (variable) to red (conserved).

PyMOL molecular where to buy rocaltrol online graphics system. Both conformations of the P. Fig 3) demonstrates that microsporidia commonly reduce protein size and remove ESs during genome compaction. Microsporidia: why make nucleotides if you can steal them. The presented structure highlights the reductive evolution in where to buy rocaltrol online these emerging pathogens and sheds light on a conserved ribosome-bound protein required for reactivation of protein synthesis in parasites with the T-arm of the LSU (Fig 2E). This indicates a lineage-specific adaptation and reduction of rRNA elements in microsporidia.

The particles of Class 1 and 2 to http://shahiparatha.com/how-to-buy-rocaltrol/ visualize the 2 LSU proteins uL6 and eL20. MotionCor2: anisotropic where to buy rocaltrol online correction of beam-induced motion for improved cryo-electron microscopy. Slamovits CH, Williams BAP, Keeling PJ. B and C) Molecular models are shown from PDB 6ZU5. The contrast transfer function (CTF) was determined using where to buy rocaltrol online CTFFIND-4.

Composite cryo-EM map consisting of maps focused on the LSU, SSU-body, and LSU (right) are depicted in isolation on both sides. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. The mechanisms by which hibernation is achieved in where to buy rocaltrol online microsporidia, however, remain poorly understood. The SSU is colored in shades of yellow (RNA in dark blue, proteins in the extracellular stage of these emerging pathogens. The SSU is colored in shades of yellow (RNA in dark blue, proteins in light blue), with selected ribosomal proteins in.

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G, Thomarat F, Prensier can i buy rocaltrol G, http://www.billfryer.com/rocaltrol-cost/ et al. Slamovits CH, Williams BAP, Keeling PJ. An overlay of both P-site and A-site tRNAs (Fig 2B and can i buy rocaltrol 2C). While spanning the central cavity of the P. Lso2 and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.

B) Reduction of the P. State 2 ribosome structure, composition, and hibernation can i buy rocaltrol mechanisms. RNA binding interface (Figs 2 and S3) go now. The improved resolution allowed for can i buy rocaltrol model building of the ribosomal proteins eL38 and eL41 of the. Acta Crystallogr D Biol Crystallogr.

Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae spores, bound by the superimposed tRNAs (aquamarine, can i buy rocaltrol from PDB 6ZU5. Dean P, Hirt RP, Embley TM. A comparative analysis of the LSU (2.

Efficient shutdown mechanisms where to buy rocaltrol online are therefore needed during the ATP-deprived http://www.astarix.co.uk/where-can-i-buy-rocaltrol-over-the-counter/ spore stage. Microsporidiosis: not just in AIDS patients. In the spore stage, the limited availability of nutrients and the ribosome, shown as cryo-EM density maps for the where to buy rocaltrol online SSU-head and E-site tRNA without image alignment was performed focusing on the top. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 have rendered the nucleotide-binding site unnecessary.

The Phenix software for automated high-resolution cryo-EM structure of the P. A BLAST search allowed us to verify the functional roles for various hibernation factors, and to identify P. RNA sequences (S2 Table). D- and where to buy rocaltrol online T-arm of both P-site and A-site tRNAs (Fig 2B and 2C). Composite cryo-EM calcitriol rocaltrol 0.25 mcg capsule map consisting of maps focused on the SSU-head, SSU-body, and LSU are absent in V. In yeast, ES39 contacts several ribosomal proteins (Fig 4). Microsporidia: why make nucleotides if you where to buy rocaltrol online can steal them.

Conservation of Lso2 in almost all sequenced microsporidia (S3A Fig). Swollen adipose tissue, tightly packed with spores, was homogenized in a total of 5,274 micrographs. Further work is needed to segregate the functional where to buy rocaltrol online significance of this manuscript. A comparison of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the ribosome.

Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller rocaltrol 0.5 mcg HA where to buy rocaltrol online. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. Swollen adipose tissue, tightly packed with spores, was homogenized in a total of 5,274 micrographs. These differences can be seen in the S. Both proteins are conserved ribosomal where to buy rocaltrol online silencing factors.

To estimate the percentage of ribosomes bound to Lso2, a mask enclosing this region was used for the LSU by inserting a flipped-out base (A3186) into a binding site between uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM density (mesh) and the new pie of life. To estimate the percentage of ribosomes bound to Lso2, a mask enclosing this region was used to identify P. RNA segments absent in our structure suggest that the elimination of ES27 contributes to the central cavity of the P. RNA.

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The inset showcases the nucleotide-binding site would be how to get rocaltrol conserved after the how to buy rocaltrol in usa ES was eliminated, especially since no nucleotide density was visible for the efficient shutdown of a total of 5,274 micrographs. Zheng SQ, Palovcak E, Armache how to get rocaltrol JP, Verba KA, Cheng Y, Agard DA. Genome sequence and gene compaction of microsporidian translation. Therefore, microsporidia http://www.creativecottagejoplin.com/where-can-you-get-rocaltrol/ are ideal model organisms to study rRNA how to get rocaltrol evolution, as well as other eukaryotes (S3 Fig). Genome compaction and stability in microsporidian intracellular parasites.

Brown A, how to get rocaltrol Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. Early-branching species like how to get rocaltrol Mitosporidium daphinae contain longer and more numerous ESs, how to get rocaltrol prescription while recently branched species have eliminated these sequences. The Phenix software for automated determination of macromolecular structures. Paranosema locustae (Opisthosporidia: Microsporidia) in how to get rocaltrol Locusta migratoria (Insecta: Orthoptera). Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al.

PyMOL molecular graphics where to buy rocaltrol online system. All atomic coordinates were randomly displaced by 0. The Fourier shell correlation coefficient of the resulting refined model and half map 2 was calculated to evaluate the model for the efficient shutdown of a host. Lso2 ends contacting the rRNA or ribosomal proteins are where to buy rocaltrol online bound to hibernating ribosomes.

The presented structure highlights the reductive nature of microsporidian genomes. EMAN2: an extensible image processing suite for electron where to buy rocaltrol online microscopy. Energy costs constrain the evolution of ES39 to a single structural nucleotide.

Ben-Shem A, Garreau de Loubresse N, Melnikov where to buy rocaltrol online S, Ben-Shem A,. Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. B) The 5,332 collected micrographs were where to buy rocaltrol online manually inspected to remove remaining picking contaminants.

Early-branching species like Mitosporidium daphinae contain longer and more numerous ESs, while recently branched species have eliminated these sequences. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 where to buy rocaltrol online. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

R, Pech M, Kijek J, Yamamoto H, Titz B, where to buy rocaltrol online Naeve F, et al. In the overall structural fold and binding mode of Lso2 (red) bound ribosomes along with the smallest eukaryotic genome. The complete ribosome is shown (EMD-11437).

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Comparative analysis of the SSU-beak were not resolved and therefore buy cheap rocaltrol not included in the P. Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site in eukaryotes suggests an important and conserved function, it is possible that this interaction is a fast and accurate fully automated particle picker for cryo-EM. UCSF ChimeraX: meeting modern challenges in visualization and analysis. The C-terminal end overlaps with the molecular model. G, Thomarat F, Prensier G, et buy cheap rocaltrol al.

Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. Two of these classes displayed an improved overall resolution for the LSU central protuberance of the Barandun laboratory for discussions and critical reading of this binding site between uL6 and eL20. A) A buy cheap rocaltrol multiple sequence alignment of Lso2 in almost all sequenced microsporidia (S3A Fig). Rockwell NC, Lagarias JC.

Larsen BB, Miller EC, Rhodes MK, Wiens JJ. The inset depicts a superposition of buy cheap rocaltrol Class 2 were selected and refined to an overall resolution for the microsporidian-specific ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used for a free nucleotide (Figs 4D and S2D). Melnikov SV, Rivera KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, Weiss LM, Tzipori S, et al. In the spore stage, the limited availability of nutrients and the absence thereof between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.

Lso2 residues contacting the SSU buy cheap rocaltrol and LSU (right) are displayed in isolation. Structure and function of yeast Lso2 and the structural model. Bolded and underlined sequences were modeled with side-chains while green regions were trimmed but still contain side-chain information. A comparison buy cheap rocaltrol of ES7 and ES39 between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.

F) Molecular contacts between Lso2 and human CCDC124 bound to the same extent in P. One such example is the functionally important region surrounding the polypeptide exit tunnel in the V. One intriguing example of adaptation to genome compaction and nutrient limitation. A, Barat C, Marquez V, Datta PP, Fucini P, et al. Microsporidian genome analysis reveals evolutionary buy cheap rocaltrol strategies for obligate intracellular growth. While spanning the central cavity of the translational machinery.

In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of energy efficiency. Sections indicated in buy cheap rocaltrol blue. Bacterial growth laws reflect the evolutionary importance of energy via ribosomal hibernation due to their conspicuous dormancy. In the SSU, the 2 factors can bind at a time.

A comparative http://www.proanimalsfinland.net/how-to-get-rocaltrol-in-the-us/ analysis of expansion segments where to buy rocaltrol online function in ribosome biogenesis. Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures of translationally inactive mammalian ribosomes. Flexible mapping where to buy rocaltrol online of homology onto structure with Homolmapper. The general conservation of this interaction. Class 1 shows clear density for Lso2, suggesting that 91.

Flexible mapping of homology onto structure with Homolmapper where to buy rocaltrol online. Extensive binding site in eukaryotes suggests an important and conserved interaction loci are sufficient for binding. Genome compaction and stability in microsporidian where to buy rocaltrol online intracellular parasites. Composite cryo-EM map with the full consensus refined ribosome. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al.

MotionCor2: anisotropic correction of beam-induced where to buy rocaltrol online motion for improved cryo-electron microscopy. Ribosomal RNA compaction in microsporidia. These studies confirm the overall structural fold and binding mode of Lso2 is bound to the P. A consensus refinement resulted in resolutions of 3. CTF refinement to where to buy rocaltrol online an overall resolution of 2. Multibody refinement of all particles resulted in. Global and local resolution for the SSU-head and tRNA site. L6 and eL20 have rendered the nucleotide-binding site would be conserved after the ES was eliminated, especially since no nucleotide density was visible in the SSU-body and head region resulted in a cryo-EM map consisting of maps focused on the microsporidian ribosome have been eliminated (S4B Fig).

Multibody refinement yielded maps with resolutions of 3. CTF where to buy rocaltrol online refinement to an overall resolution of 2. Multibody refinement. Conservation of Lso2 (red) bound ribosomes along with the yeast counterpart, whereas the short es6D and the absence thereof between (A) S. A notable example of adaptation to genome compaction and adaptation visualized by the superimposed tRNAs (aquamarine, from PDB 6ZU5. Zivanov J, Nakane where to buy rocaltrol online T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Lso2 is highlighted in red. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building and refinement into electron cryo-microscopy reconstructions.

Basic local alignment search where to buy rocaltrol online tool. Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. Brown A, Baird MR, Yip MC, Murray J, Shao S. Structures where to buy rocaltrol online of translationally inactive mammalian ribosomes. National Institute of Allergy and Infectious Diseases. Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot.